Generator

Part:BBa_K1179022

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)

CAG_Acyl-TyA-rtTA3

This part is the constitutive expression of Acyl-TyA-rtTA, which is exported out of the cell and into exosomes


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2373
    Illegal PstI site found at 2622
    Illegal PstI site found at 2644
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
    Illegal PstI site found at 2373
    Illegal PstI site found at 2622
    Illegal PstI site found at 2644
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal BglII site found at 2473
    Illegal XhoI site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2373
    Illegal PstI site found at 2622
    Illegal PstI site found at 2644
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2373
    Illegal PstI site found at 2622
    Illegal PstI site found at 2644
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None